#12: Killer Croc

#11: Lock-Up

#10: The Ventriloquist and Scarface

#9: Clay-Face

#8: The Scarecrow

#7: The Mad Hatter

#6: The Riddler

#5: Harley Quinn

#4: The Joker

#3: Baby Doll

#2: Two-Face

#1: Mr. Freeze

Honorable Mentions:
The Penguin

Man-Bat

Roland Dagget

Rubert Thorne

Red Claw

Catwoman

Poison Ivy

Raz Al Ghul

Captain Clown

The Phantasm